Priming with rAAV encoding RBD of SARS-CoV S protein and boosting with RBD-specific peptides for T cell epitopes elevated humoral and cellular immune responses against SARS-CoV infection.
Identifieur interne : 003054 ( Main/Exploration ); précédent : 003053; suivant : 003055Priming with rAAV encoding RBD of SARS-CoV S protein and boosting with RBD-specific peptides for T cell epitopes elevated humoral and cellular immune responses against SARS-CoV infection.
Auteurs : Lanying Du [République populaire de Chine] ; Guangyu Zhao ; Yongping Lin ; Chris Chan ; Yuxian He ; Shibo Jiang ; Changyou Wu ; Dong-Yan Jin ; Kwok-Yung Yuen ; Yusen Zhou ; Bo-Jian ZhengSource :
- Vaccine [ 0264-410X ] ; 2008.
Descripteurs français
- KwdFr :
- Animaux, Calendrier vaccinal, Cytométrie en flux, Dependovirus (génétique), Dependovirus (immunologie), Femelle, Immunité cellulaire (immunologie), Immunoglobuline G (analyse), Immunoglobuline G (biosynthèse), Lymphocytes T (immunologie), Lymphocytes T CD4+ (immunologie), Lymphocytes T CD8+ (immunologie), Production d'anticorps (immunologie), Protéines de la matrice virale (génétique), Protéines de la matrice virale (immunologie), RT-PCR, Rappel de vaccin, Récepteurs viraux (génétique), Récepteurs viraux (immunologie), Souris, Souris de lignée BALB C, Syndrome respiratoire aigu sévère (), Syndrome respiratoire aigu sévère (immunologie), Syndrome respiratoire aigu sévère (virologie), Test ELISA, Tests de neutralisation, Vaccins antiviraux (immunologie), Vaccins synthétiques (immunologie), Virus du SRAS (immunologie), Épitopes (immunologie).
- MESH :
- analyse : Immunoglobuline G.
- biosynthèse : Immunoglobuline G.
- génétique : Dependovirus, Protéines de la matrice virale, Récepteurs viraux.
- immunologie : Dependovirus, Immunité cellulaire, Lymphocytes T, Lymphocytes T CD4+, Lymphocytes T CD8+, Production d'anticorps, Protéines de la matrice virale, Récepteurs viraux, Syndrome respiratoire aigu sévère, Vaccins antiviraux, Vaccins synthétiques, Virus du SRAS, Épitopes.
- virologie : Syndrome respiratoire aigu sévère.
- Animaux, Calendrier vaccinal, Cytométrie en flux, Femelle, RT-PCR, Rappel de vaccin, Souris, Souris de lignée BALB C, Syndrome respiratoire aigu sévère, Test ELISA, Tests de neutralisation.
English descriptors
- KwdEn :
- Animals, Antibody Formation (immunology), CD4-Positive T-Lymphocytes (immunology), CD8-Positive T-Lymphocytes (immunology), Dependovirus (genetics), Dependovirus (immunology), Enzyme-Linked Immunosorbent Assay, Epitopes (immunology), Female, Flow Cytometry, Immunity, Cellular (immunology), Immunization Schedule, Immunization, Secondary, Immunoglobulin G (analysis), Immunoglobulin G (biosynthesis), Mice, Mice, Inbred BALB C, Neutralization Tests, Receptors, Virus (genetics), Receptors, Virus (immunology), Reverse Transcriptase Polymerase Chain Reaction, SARS Virus (immunology), Severe Acute Respiratory Syndrome (immunology), Severe Acute Respiratory Syndrome (prevention & control), Severe Acute Respiratory Syndrome (virology), T-Lymphocytes (immunology), Vaccines, Synthetic (immunology), Viral Matrix Proteins (genetics), Viral Matrix Proteins (immunology), Viral Vaccines (immunology).
- MESH :
- chemical , analysis : Immunoglobulin G.
- chemical , biosynthesis : Immunoglobulin G.
- chemical , genetics : Receptors, Virus, Viral Matrix Proteins.
- chemical , immunology : Epitopes, Receptors, Virus, Vaccines, Synthetic, Viral Matrix Proteins, Viral Vaccines.
- genetics : Dependovirus.
- immunology : Antibody Formation, CD4-Positive T-Lymphocytes, CD8-Positive T-Lymphocytes, Dependovirus, Immunity, Cellular, SARS Virus, Severe Acute Respiratory Syndrome, T-Lymphocytes.
- prevention & control : Severe Acute Respiratory Syndrome.
- virology : Severe Acute Respiratory Syndrome.
- Animals, Enzyme-Linked Immunosorbent Assay, Female, Flow Cytometry, Immunization Schedule, Immunization, Secondary, Mice, Mice, Inbred BALB C, Neutralization Tests, Reverse Transcriptase Polymerase Chain Reaction.
Abstract
Development of vaccines against severe acute respiratory syndrome (SARS) coronavirus (SARS-CoV) is crucial in the prevention of SARS reemergence. The receptor-binding domain (RBD) of SARS-CoV spike (S) protein is an important target in developing safe and effective SARS vaccines. Our previous study has demonstrated that vaccination with adeno-associated virus encoding RBD (RBD-rAAV) induces high titer of neutralizing antibodies. In this study, we further assessed the immune responses and protective effect of the immunization with RBD-rAAV prime/RBD-specific T cell peptide boost. Compared with the RBD-rAAV prime/boost vaccination, RBD-rAAV prime/RBD-peptide (RBD-Pep) boost induced similar levels of Th1 and neutralizing antibody responses that protected the vaccinated mice from subsequent SARS-CoV challenge, but stronger Th2 and CTL responses. No significant immune responses and protective effects were detected in mice vaccinated with RBD-Pep or blank AAV alone. Since T cell epitopes are highly conserved and boosting with peptides may induce the production of effector memory T cells, which may be effective against viruses with mutations in the neutralizing epitopes, our results suggest that the vaccination protocol used may be ideal for providing effective, broad and long-term protection against SARS-CoV infection.
DOI: 10.1016/j.vaccine.2008.01.025
PubMed: 18289745
Affiliations:
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Le document en format XML
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last="Lin">Yongping Lin</name></author><author><name sortKey="Chan, Chris" sort="Chan, Chris" uniqKey="Chan C" first="Chris" last="Chan">Chris Chan</name></author><author><name sortKey="He, Yuxian" sort="He, Yuxian" uniqKey="He Y" first="Yuxian" last="He">Yuxian He</name></author><author><name sortKey="Jiang, Shibo" sort="Jiang, Shibo" uniqKey="Jiang S" first="Shibo" last="Jiang">Shibo Jiang</name></author><author><name sortKey="Wu, Changyou" sort="Wu, Changyou" uniqKey="Wu C" first="Changyou" last="Wu">Changyou Wu</name></author><author><name sortKey="Jin, Dong Yan" sort="Jin, Dong Yan" uniqKey="Jin D" first="Dong-Yan" last="Jin">Dong-Yan Jin</name></author><author><name sortKey="Yuen, Kwok Yung" sort="Yuen, Kwok Yung" uniqKey="Yuen K" first="Kwok-Yung" last="Yuen">Kwok-Yung Yuen</name></author><author><name sortKey="Zhou, Yusen" sort="Zhou, Yusen" uniqKey="Zhou Y" first="Yusen" last="Zhou">Yusen Zhou</name></author><author><name sortKey="Zheng, Bo Jian" sort="Zheng, Bo Jian" 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G" first="Guangyu" last="Zhao">Guangyu Zhao</name></author><author><name sortKey="Lin, Yongping" sort="Lin, Yongping" uniqKey="Lin Y" first="Yongping" last="Lin">Yongping Lin</name></author><author><name sortKey="Chan, Chris" sort="Chan, Chris" uniqKey="Chan C" first="Chris" last="Chan">Chris Chan</name></author><author><name sortKey="He, Yuxian" sort="He, Yuxian" uniqKey="He Y" first="Yuxian" last="He">Yuxian He</name></author><author><name sortKey="Jiang, Shibo" sort="Jiang, Shibo" uniqKey="Jiang S" first="Shibo" last="Jiang">Shibo Jiang</name></author><author><name sortKey="Wu, Changyou" sort="Wu, Changyou" uniqKey="Wu C" first="Changyou" last="Wu">Changyou Wu</name></author><author><name sortKey="Jin, Dong Yan" sort="Jin, Dong Yan" uniqKey="Jin D" first="Dong-Yan" last="Jin">Dong-Yan Jin</name></author><author><name sortKey="Yuen, Kwok Yung" sort="Yuen, Kwok Yung" uniqKey="Yuen K" first="Kwok-Yung" last="Yuen">Kwok-Yung Yuen</name></author><author><name sortKey="Zhou, Yusen" 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ELISA</term><term>Tests de neutralisation</term></keywords></textClass></profileDesc></teiHeader><front><div type="abstract" xml:lang="en">Development of vaccines against severe acute respiratory syndrome (SARS) coronavirus (SARS-CoV) is crucial in the prevention of SARS reemergence. The receptor-binding domain (RBD) of SARS-CoV spike (S) protein is an important target in developing safe and effective SARS vaccines. Our previous study has demonstrated that vaccination with adeno-associated virus encoding RBD (RBD-rAAV) induces high titer of neutralizing antibodies. In this study, we further assessed the immune responses and protective effect of the immunization with RBD-rAAV prime/RBD-specific T cell peptide boost. Compared with the RBD-rAAV prime/boost vaccination, RBD-rAAV prime/RBD-peptide (RBD-Pep) boost induced similar levels of Th1 and neutralizing antibody responses that protected the vaccinated mice from subsequent SARS-CoV challenge, but stronger Th2 and CTL responses. No significant immune responses and protective effects were detected in mice vaccinated with RBD-Pep or blank AAV alone. Since T cell epitopes are highly conserved and boosting with peptides may induce the production of effector memory T cells, which may be effective against viruses with mutations in the neutralizing epitopes, our results suggest that the vaccination protocol used may be ideal for providing effective, broad and long-term protection against SARS-CoV infection.</div></front></TEI><affiliations><list><country><li>République populaire de Chine</li></country></list><tree><noCountry><name sortKey="Chan, Chris" sort="Chan, Chris" uniqKey="Chan C" first="Chris" last="Chan">Chris Chan</name><name sortKey="He, Yuxian" sort="He, Yuxian" uniqKey="He Y" first="Yuxian" last="He">Yuxian He</name><name sortKey="Jiang, Shibo" sort="Jiang, Shibo" uniqKey="Jiang S" first="Shibo" last="Jiang">Shibo Jiang</name><name sortKey="Jin, Dong Yan" sort="Jin, Dong Yan" uniqKey="Jin D" first="Dong-Yan" last="Jin">Dong-Yan Jin</name><name sortKey="Lin, Yongping" sort="Lin, Yongping" uniqKey="Lin Y" first="Yongping" last="Lin">Yongping Lin</name><name sortKey="Wu, Changyou" sort="Wu, Changyou" uniqKey="Wu C" first="Changyou" last="Wu">Changyou Wu</name><name sortKey="Yuen, Kwok Yung" sort="Yuen, Kwok Yung" uniqKey="Yuen K" first="Kwok-Yung" last="Yuen">Kwok-Yung Yuen</name><name sortKey="Zhao, Guangyu" sort="Zhao, Guangyu" uniqKey="Zhao G" first="Guangyu" last="Zhao">Guangyu Zhao</name><name sortKey="Zheng, Bo Jian" sort="Zheng, Bo Jian" uniqKey="Zheng B" first="Bo-Jian" last="Zheng">Bo-Jian Zheng</name><name sortKey="Zhou, Yusen" sort="Zhou, Yusen" uniqKey="Zhou Y" first="Yusen" last="Zhou">Yusen Zhou</name></noCountry><country name="République populaire de Chine"><noRegion><name sortKey="Du, Lanying" sort="Du, Lanying" uniqKey="Du L" first="Lanying" last="Du">Lanying Du</name></noRegion></country></tree></affiliations></record>Pour manipuler ce document sous Unix (Dilib)
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